Module Topics

Topic	Main Teaching Methods	Lecture Hours	Laboratory Hours
Embryology & physiology of the male & female reproductive Systems	Lecture	3	0
Evaluation & Preparation of Sperm	Lecture; Practical Lab	1	2
Endocrinology in the context of reproductive systems	Lecture	3	0
Review of cell biology, molecular biology, & cell-cell interactions	Lecture	3	0
Microbiology & ART	Practical Lab	0	3
The oocyte & oogenesis	Lecture	3	0
Gametogenesis & Spermatogenesis	Lecture	3	0
Embryo development	Lecture	3	0
Preparation and evaluation of oocytes	Lecture; Practical Lab	1	2
Designing the IVF Lab	Lecture	3	0
Sperm Function Tests	Lecture; Practical Lab	1	2
Post Implantation embryology	Lecture	3	0
Oocyte retrieval selection	Lecture; Practical Lab	1	2
IVF Laboratory Management	Lecture	3	0
Advanced topics in sperm selection & injection (Hyaluronic acid binding-mediated sperm)	Lecture; Practical Lab	1	2
Quality Control in IVF Laboratory	Lecture; Case Studies	1	2
	Total Contact Hours	39	21

Topic	Main Teaching Methods	Lecture Hours	Laboratory Hours
Introduction to Micromanipulation	Lecture	3	0
Physics of micromanipulation	Lecture	3	0
Intracytoplasmic sperm injection (ICSI)	Lecture	3	0
Equipment for ICSI	Practical Lab	0	3
Practical techniques in ICSI	Practical Lab	0	3
Media required to perform ICSI	Lecture; Practical Lab	1	2
Assisted Hatching	Lecture; Practical Lab	1	2
Denuding of oocyte & Micropipette handling	Lecture	0	3
Sperm immobilization	Lecture; Practical Lab	1	2
Practical session on micropipettes and sperm immobilization	Practical Lab	0	3
Preparation of sperm for ICSI	Lecture; Practical Lab	1	2
Cytogenetics & Chromosomal and Genetic Analysis in IVF	Lecture	2	2
Indication and contraindication of ICSI procedure	Lecture	2	0
azoospermia and icsi	Lecture	3	0
Intracytoplasmic morphologically selected sperm (IMSI)	Lecture; Practical Lab	1	2
Introduction to PESA, TESA, & TESE	Lecture	3	0
Practical sessions on PESA, TESA, & TESE	Practical Lab	0	3
Identification of abnormal & immature sperm	Lecture; Practical Lab	1	2
Identification of spermatids, spermatocytes and other cells	Lecture; Practical Lab	1	2
Practical sessions and review on ICSI and micromanipulation	Practical Lab	0	3
	Total	26	34

Topic	Main Teaching Methods	Lecture Hours	Laboratory Hours
Analysis of fertilization	Lecture
Culture systems for the human embryo	Lecture; Practical Lab
Handling and culture techniques	Lecture; Practical Lab
Sequential culture media & buffer	Lecture; Practical Lab	3	0
Analysis of morphology and quantification of nutrient utilization and the metabolome	Lecture; Practical Lab	1	2
Time-lapse imaging to assess embryo morphokinesi	Lecture; Practical Lab	1	2
Selection of embryo	Lecture; Practical Lab	1	2
Blastocyst culture techniques	Lecture; Practical Lab	1	2
Embryo transfer techniques	Lecture; Practical Lab	1	2
Embryo Reduction	Lecture; Practical Lab	1	2

Topic	Main Teaching Methods	Lecture Hours
Introduction to ethics in ART & IVF	Lecture; case studies; role playing	3
Ethical considerations and moral dilemmas in ART	Lecture	3
Regulations and legal frameworks on ART and IVF in Egypt	Lecture; case studies; role playing	3
Patient counseling for ART & IVF	Lecture; case studies; role playing	3
Patient consent and informed consent	Lecture; case studies; role playing	3
Legal and ethical considerations for surrogacy	Lecture; case studies; role playing	3
Clear and effective communication: Risks and success rates of ART & IVF	Lecture; case studies; role playing	3
Clear and effective communication: Handling patient inquiries, concerns, & beliefs	Lecture; case studies; role playing	3
Clear and effective communication: Simplifying language and communication methods for patients with limited educational backgrounds	Lecture; case studies; role playing	3
Social responsibility of medical professionals and laboratories	Lecture	3
	Total	30

Topic	Main Teaching Methods	Lecture Hours	Laboratory Hours
Lab set-up for cryopreservation	Practical Lab	0	3
Fundamentals of cryobiology	Lecture	3	0
Physiology of cryobiology	Lecture	3	0
Cryoprotectants & additives	Lecture; Practical Lab	1	2
Equipment for cryopreservation	Lecture; Practical Lab	1	2
Controlled Rate Cooling & Slow Freezing	Lecture; Practical Lab	1	2
Embryo thawing	Lecture; Practical Lab	1	2
Oocyte & Ovarian freezing/thawing	Lecture; Practical Lab	1	2
sperm freezing & Testicular freezing/thawing	Lecture; Practical Lab	1	2
Frozen Embryo Transfer	Lecture; Practical Lab	1	2
Recent developments in cryobiology	Lecture	3	0
Monitoring and timing of the FET cycle	Lecture	3	0
Quality assurance in cryopreservation	Lecture; Practical Lab	2	1
Review of cryopreservation and the advantages and disadvantages of different methods	Lecture	3	0
	Total	27	18

Topic	Main Teaching Methods	Lecture Hours	Laboratory Hours
Preimplantation Genetic Testing (PGT) Technologies	Lecture; Practical Lab	1	2
Typical Protocols for Embryo Biopsy	Lecture	3	1
Commercial PGT Services	Lecture	2	0
Methods of Genetic Analysis for PGT Following Embryo or Polar Body Biopsy (PCR & FISH)	Lecture; Practical Lab	1	2
Single Cell Genomics & PGT	Lecture; Practical Lab	1	2
Methods of Whole-Genome Amplification (WGA)	Lecture; Practical Lab	1	2
Stem Cells: Properties, Pluripotent Stem Cells (PSCs), Embryonic Stem Cells (ESCs), & Induced PSCs (iPSCs)	Lecture	3	0
Therapeutic Applications of Stem Cells	Lecture	2	0
Artificial Gametes from Spermatogonial/Oogonial Stem Cells	Lecture; Practical Lab	1	2
Artificial Gametes from Pluripotent Stem Cells	Lecture; Practical Lab	1	2
Epigenetics & ART	Lecture; Practical Lab	1	2
ART-induced Epigenetic Diseases	Lecture	1	2
Infertility and Epigenetics	Lecture	3	0
Non-Genetic Factors of Epigenetic Programing	Lecture	3	0
Practical Session & Module Review	Lecture; Practical Lab	2	2
	Total	26	19

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